Understanding Cetrotide: Injection Instructions and Information

Note: This article is written by a professional healthcare provider for informational purposes. Please follow your doctor’s instructions and refer to the official package leaflet for your medication.


Cetrotide 0.25 mg, a medication developed by Merck, is an important component in assisted reproductive techniques. It is used to help stimulate the ovaries to produce more eggs, increasing the chances of successful conception. This article will provide you with a clear understanding of Cetrotide, how to use it, potential side effects, and essential information for safe and effective administration.

What is Cetrotide?

Cetrotide contains the active substance cetrorelix acetate and is designed to halt the premature release of eggs from the ovaries during the menstrual cycle. This is a crucial step in fertility treatments, ensuring that eggs are not released too early, which could interfere with the treatment’s success.

How to Use Cetrotide

Here’s a step-by-step guide on how to properly prepare and administer Cetrotide:

  1. Preparation: Make sure the medication has reached room temperature by taking it out of the refrigerator approximately 30 minutes before use.
  2. Hand Hygiene: Wash your hands thoroughly before handling the medication.
  3. Gather Supplies: Lay out all the necessary items on a clean, flat surface. These include:
    • One vial of Cetrotide powder
    • One pre-filled syringe containing sterile water (solvent)
    • Two needles: one with a yellow mark for mixing the solution and one with a grey mark for injection
    • Two alcohol swabs
  4. Mixing the Solution:
    • Remove the cap from the vial and clean the rubber stopper and metal ring using the first alcohol swab.
    • Take the syringe with the yellow-marked needle and inject the sterile water into the vial. Do not shake it; gentle swirling is sufficient. The solution should become clear and free from particles.
    • To remove air bubbles, gently tap the syringe to collect the air at the top, then push the plunger to eliminate them.
  5. Choosing an Injection Site:
    • Select an injection site on your belly. Rotating the injection site can help reduce skin irritation.
    • Clean the chosen area using your second alcohol swab, applying it in a circular motion.
  6. Injecting Cetrotide:
    • Hold the syringe with the grey-marked needle at an angle of 45 to 90 degrees.
    • Slowly insert the needle into your skin.
    • If blood appears upon insertion, refer to step 7.
    • If no blood appears, inject the medicine slowly and steadily.
  7. Handling Blood in the Syringe:
    • If you notice blood when inserting the needle, remove the needle slowly and apply slight pressure with the alcohol swab.
    • Dispose of the medicine and prepare a new dose, starting from step 1.
  8. Safe Disposal:
    • Once used, dispose of each needle, vial, and syringe properly. Consult your pharmacist for safe disposal methods.

It’s crucial to follow these steps diligently and pay close attention to hygiene to ensure the effectiveness of the medication.

Possible Side Effects

As with any medication, Cetrotide may lead to side effects in some individuals. Here are some potential side effects:

  • Mild and short-lasting skin irritation at the injection site, such as redness, itching, or swelling.
  • Feeling sick (nausea).
  • Headache.
  • Lower abdominal pain, accompanied by nausea or vomiting, which may indicate Ovarian Hyper-stimulation Syndrome (OHSS).

If you notice any of these side effects, it’s essential to stop using Cetrotide and contact your healthcare provider immediately.

Additional Information

  1. Allergies: Inform your doctor if you have allergies or a history of allergic reactions before using Cetrotide.
  2. Liver and Kidney Disease: If you have liver or kidney disease, discuss this with your doctor as Cetrotide has not been studied extensively in such cases.
  3. Pregnancy and Breastfeeding: Cetrotide should not be used during pregnancy or breastfeeding.
  4. Repeat Cycles: Experience with using Cetrotide for more than one cycle is limited. Your doctor will weigh the benefits and risks if multiple cycles are necessary.
  5. Driving and Machines: Cetrotide is not expected to affect your ability to drive or operate machinery.


Cetrotide plays a vital role in fertility treatments by preventing premature ovulation. When using Cetrotide, ensure strict adherence to the preparation and injection instructions. In case of side effects or concerns, contact your healthcare provider promptly. Follow their guidance throughout your treatment process, and remember that effective communication with your doctor is key to a successful fertility journey.

Please note that this article serves as a general guide and should not replace the instructions provided by your healthcare professional.